Pure MMB-4en-PICA Available
What is MMB-022?
Product Information, Pure MMB-022 Online
Appearance: White crystalline
Application: Synthetic pharmaceutical intermediates
Purity: 99% min
MMB-022 (methyl 3-methyl-2-[1-(pent-4-en-1-yl)-1H-indole-3-carboxamido]butanoate) is a new synthetic cannabinoid with an alkene at the pentenyl side chain, a rare functional group for synthetic cannabinoids.
Metabolite identification is an important step for detection of synthetic cannabinoids in humans, since they are generally extensively metabolise.
The aims of the study were to tentatively identify in vitro phase I metabolites, to confirm major metabolites using synthesize metabolites, to examine metabolic pathways thoroughly, to study metabolic stability and to suggest metabolites appropriate for urine screening.
MMB022 and its synthesize metabolites were put in an incubator with human liver microsomes (HLM) and the supernatants analyze by liquid chromatography-quadrupole time-of-flight mass spectrometry.
As a result sixteen metabolites were identify, which were generate via dehydrogenation, dihydrodiol formation, ester hydrolysis, hydroxylation, and combinations thereof.
A major biotransformation of the alkene at the pentenyl side chain was confirm to be dihydrodiol formation.
Also the major metabolites were ester hydrolysis (M15) and dihydrodiol (M8) metabolites.
whereas the metabolite derived from the combination of ester hydrolysis and also dihydrodiol (M5) was the 4th most abundant metabolite.
Above all the metabolic pathways were investigate using synthesize metabolites and so, reveal that M5 is an end product of the pathways, indicating that it might become a more abundant metabolite in vivo depending on the rate of metabolism in humans.
The major pathway of MMB022 to M5 were determine to be via M8 formation.
However intrinsic clearance of MMB022 was also determine to be 296 mL/min/kg and t1/2 was 2.1 min indicating a low metabolic stability.
M15, M8 and potentially M5 are suitable urinary targets.